Project

ELI: Electronic Life-detection Instrument

Copyright

Di Ventra & Taniguchi, 2016

Di Ventra & Taniguchi, 2016

Life on Mars, if it exists, could have been transferred between Earth and Mars due to meteorite impacts. The Search for Extra-Terrestrial Genomes (SETG; setg.mit.edu) is under development to enable the search for nucleic acid (DNA, RNA) based life on Earth and for diverse other space-related applications (e.g., Carr et al. 2017). In our prior ZeroG experiment (November 2017) we demonstrated a robust sequencing capability during parabolic flight using a small commercial device (Oxford Nanopore MinION Mk-1B) weighing ~ 100 g and powered by a USB port. This device detects double stranded DNA or single stranded RNA after appropriate library preparation, which consists of many enzymatic steps to prepare the nucleic acids (DNA, RNA) for sequencing. Life beyond Earth may or may not use standard nucleic acids, thus, the astrobiology community seeks devices capable of detecting not only DNA or RNA, but nucleic acids or other informational polymers. In addition, targeting other biomolecules that are stable over geologic time, such as amino acids, is highly desirable.

Here we propose to test an element of the Electronic Life-detec… View full description

Life on Mars, if it exists, could have been transferred between Earth and Mars due to meteorite impacts. The Search for Extra-Terrestrial Genomes (SETG; setg.mit.edu) is under development to enable the search for nucleic acid (DNA, RNA) based life on Earth and for diverse other space-related applications (e.g., Carr et al. 2017). In our prior ZeroG experiment (November 2017) we demonstrated a robust sequencing capability during parabolic flight using a small commercial device (Oxford Nanopore MinION Mk-1B) weighing ~ 100 g and powered by a USB port. This device detects double stranded DNA or single stranded RNA after appropriate library preparation, which consists of many enzymatic steps to prepare the nucleic acids (DNA, RNA) for sequencing. Life beyond Earth may or may not use standard nucleic acids, thus, the astrobiology community seeks devices capable of detecting not only DNA or RNA, but nucleic acids or other informational polymers. In addition, targeting other biomolecules that are stable over geologic time, such as amino acids, is highly desirable.

Here we propose to test an element of the Electronic Life-detection Instrument (ELI), specifically a solid-state single molecule detector. We propose to target amino acids and IPs, including nucleic acids, though note that, in principal, many other types of molecules could be detected. This versatility will support unambiguous life detection and detection of forward contamination. ELI relies on solid-state quantum electronic tunneling (QET) nanogap sensors (Fig. 1), which can detect and discriminate among single amino acids, and detect RNA and DNA, including bases and (in a very limited fashion) sequences.

The research goals of the experiment are to 1) Quantify the impact of g-level on the nanogap device, and 2) Quantify changes in noise due to g-level and vibration. In addition, we aim to perform single molecule detection of amino acids.

Research Topics
#space #technology #zero gravity

Copyright

Di Ventra & Taniguchi, 2016

Copyright

Chris Carr

Team Members

Christopher E. Carr, Principal Investigator

Maria T. Zuber, Co-Investigator

Gary Ruvkun, Co-Investigator

Jason Soderblom, Co-Investigator

Jack Szostak, Co-Investigator

Masateru Taniguchi, Co-Investigator

Daniel Duzdevich, Postdoctoral Associate

Delson Faria Dasilva, Undergraduate Researcher

Spencer Meek, Summer High School Intern

Acknowledgements

MIT Media Lab Space Exploration Initiative (Zero-G Flight)

Funding: NASA 80NSSC19K1028

References

Carr CE, Mojarro A, Hachey J, Saboda K, Tani J, Bhattaru SA, Smith A, Pontefract A, Zuber MT, Finney M, Doebler R, Brown M, Talbot R, Nguyen V, Bailey R, Ferguson T, Church G, Ruvkun G. Towards In Situ Sequencing for Life Detection. Aerospace Conference, 2017 IEEE. March 4-11, Big Sky, MT, USA, pp. 1-18. Session 2.07 In Situ Instruments for Landed Surface Exploration, Orbiters and Flybys. Paper # 2353 doi:10.1109/AERO.2017.7943896

Di Ventra, M. & Taniguchi, M. in Nat Nano Vol. 11 117-126 (Nature Publishing Group, 2016).