Dissertation Title: Highly multiplexed molecular mapping of biological samples via integrated experimental and computational technologies

Abstract: 

Identification and labeling of diverse molecular identities in biological samples has traditionally come at a price of decreased spatial information. Electron microscopy, with its nanometer spatial resolution and excellent ultrastructure preservation, yet essentially no molecular identification, is the most obvious example of this dichotomy that pervades all imaging paradigms. Our lab has previously developed Expansion Microscopy (ExM), which increases both spatial resolution of fluorescence imaging and target accessibility via isotropically expandable hydrogels. While proteomic-based approaches have the bottleneck of antibodies, nucleic acid sequencing is universally applicable to every molecular target and provides for uniform sample handling and essentially infinite multiplexing. In this thesis, I present the development of the Expansion Sequencing (ExSeq) technology suite, which resolves the underlying tensions between molecular, spatial, and ultrastructural information by multiplexing in situ sequencing and protein information in single, intact specimens. ExSeq produces high-resolution transcriptomic maps of intact tissues and is sensitive enough to detect thousands of different genes within a single sample. Applied to the mouse hippocampus, ExSeq produces transcriptomic atlases of diverse cell types and visualizes mRNA transcript content across thousands of dendritic spines and shafts of single CA1 pyramidal neurons. ExSeq also reveals the molecular organization and position-dependent state transitions of many cells in a human metastatic breast cancer sample from a patient. ExSeq harnesses novel techniques in both experimental and computational approaches to systematically encode and decode biological information from the microscope. I conclude with an exploration of ExSeq as a platform technology for molecular connectomics, with an eye toward robust and democratizeable synaptic-scale maps of the brain.

Committee members: 

Edward Boyden, Professor of Media Arts and Sciences, MIT
George Church, Professor of Genetics, Harvard Medical School
Sebastian Seung, Professor of Neuroscience, Princeton University